Abstract

Author(s): Maryvonne ARDOUREL, Khaoula RAMCHANI-BEN OTHMAN, Amir ATTALLAH, Fabien LESNE, Stéphane GRÉGOIRE, Niyazi ACAR, Olivier PERCHE* and Isabelle Ranchon COLE

Purpose: Dietary supplement enriched with antioxidants and fish oil had been shown to protect the retina from oxidative stress in vivo from light-induced retinal damage as well as Müller cells in vitro from H₂O₂ treatment. The objective of the present study was to evaluate the effect of this complex supplement on human retinal pigment epithelium cells line (Arising Retinal Pigment Epithelia- ARPE-19) since RPE (Retinal Pigment Epithelia) is key for nutrients uptake from the choriocapillaris and to the photoreceptors of neural retina.

Methods: We used ARPE-19 cells treated for 7 days with the dietary supplement at 11 or 44 μM eq. DHA.

Results: Herein, we demonstrated that incubation of ARPE-19 cells for 7 days with the supplement significantly reduced cells death induced by H₂O₂-oxidative stress from 11 μM and 44 μM equivalent DHA. Interestingly, we showed different mechanism of protection at both concentrations with a direct signal effect at 11 μM and through membrane composition modification at 44 μM. Surprisingly, at 44 μM equivalent DHA, the supplement induces phosphatidylserine externalization, an increase of isoprostanes production and caspase-3/7 activation.

Conclusion: At this high concentration, pre-conditioning effect seems to take place. Nevertheless, these results raised up the question of the long-term treatment in human.